Thesis (Ph.D) - University of Birmingham, Department of Chemical Engineering, 1996.
|Statement||by Jonathan George Huddleston.|
|The Physical Object|
Aqueous two-phase systems are generated by mixing aqueous solutions of two water-soluble polymers, or a polymer and a salt. These systems offer extremely mild conditions for separation of cells, organelles, proteins and other biomolecules, in biochemical processes. Considerable attention has been directed towards the use of the two-phase systems in several areas of by: from book Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products (pp) Aqueous Two-Phase Systems for the Recovery . Aqueous two-phase system (ATPS) is a liquid–liquid separation technique that has shown great potential for the extraction, recovery, and purification of a . Jue E, Yamanishi CD, Chiu RYT, Wu BM, Kamei DT. Using an aqueous two-phase polymer-salt system to rapidly concentrate viruses for improving the detection limit of the lateral-flow immunoassay. Biotechnol Bioeng. ;(12)– CrossRef Google Scholar.
Aqueous two-phase system (ATPS) is a liquid–liquid fractionation method. The method is based on incom-patibility of two aqueous solutions such as a polymer/ salt system [e.g. polyethylene glycol (PEG) and potassium phosphate], a polymer/polymer system (e.g. PEG/dex-tran) (Show et al. ; Rosa et al. ), an ionic liquidCited by: Huddleston J.G., Lyddiatt A. () Aqueous Two-Phase Partition in Biochemical Recovery from Mammalian Cell Culture. In: Fisher D., Sutherland I.A. (eds) Separations Using Aqueous Phase Systems. Springer, Boston, MA. aqueous two-phase systems and mass transfer mode of solvent sublation, has been successfully applied for the separation and puriﬁcation of biological materials in the ﬁeld of biotechnology. Aqueous two phase system (ATPS) is a liquid – liquid extraction method which employs two aqueous phases having applications in the field of biotechnology for the separation and purification of biological materials such as proteins, enzymes, nucleic acids, virus, antibodies and cell organelles. This review discusses the basic principles of ATPS, the factors affecting partitioning.
Yield recovery from top phase (R T) and from bottom phase (RB) are calculated in order to evaluate the purification ratio (PR) from aqueous two phase system, according to following equations: 1 1 T V R RK = + (5) 1 B V R R K = + (6) Recovery of target protein Recovery of total protein. During the last 60 years, aqueous two-phase systems (ATPS) have been the focus of much research. A large number of studies have been reported on the characterization of these systems and their use on separation, analytic, and biotechnological processes. On this review an overview about the ATPS fundamentals and properties is provided. Gholam Reza Pazuki, Vahid Taghikhani, Manuchehr Vossoughi, A Modified Local Composition-Based Model for Correlating the Vapor-Liquid and Liquid-Liquid Phase Equilibria of Aqueous Polymer-Salt Systems, Journal of Solution Chemistry, /s, 37, 5, (), (). Aqueous two-phase systems may form on mixing pairs or multiples of polymers in solution (polymer- polymer system) or between mixtures of polymers and salts (polymer--salt system). There are similarities and differences between these two principal forms of aqueous two-phase systems.